SEQUENCE_ID=template_mis_1
PRIMER_NUM_RETURN=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTG
=
SEQUENCE_ID=template_mis_2
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTG
=
SEQUENCE_ID=template_mis_3
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatgcgtacgatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCC
=
SEQUENCE_ID=template_mis_4
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACSCCCCCCCCCCCCCCCCA
=
SEQUENCE_ID=template_mis_5
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatTCCTACAGCTGTGGGAAAATGatgcgtacgatccatgctagctagctactatcgattagctatcatctactatcatctactatctactacacatctactatcatctacacacacacacacactaggccgctatatcatctagcGCCGCCATGTACACACTGatatcatctagcGCCGCCATGTACACACTGCCCCCCCCCCCCCCCCCCACTCCCCCCCCCCCCCCCCA
=
SEQUENCE_ID=template_mis_6
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCACCCCCCCCACCCCCCCCCCCCCCCCCC
=
SEQUENCE_ID=template_mis_7
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=TCCTACAGCTGTGGGAAAATGat
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
=
SEQUENCE_ID=template_mis_8
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=11
PRIMER_MAX_POLY_X=40
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
SEQUENCE_PRIMER_REVCOMP=GGGGGGGGGGGGGGGGGG
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
P3_COMMENT=Should succeed
=
SEQUENCE_ID=template_mis_9
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=9
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_COMPL_END=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
P3_COMMENT=Should succeed
=
SEQUENCE_ID=template_mis_10
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_TEMPLATE_MISPRIMING=6.7
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=100
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_LIBERAL_BASE=1
PRIMER_PRODUCT_SIZE_RANGE=36-300
PRIMER_MAX_GC=100
SEQUENCE_TEMPLATE=TCCTACAGCTGTGGGAAAATGatNgcgtacNCCCCCCCCCAAAAAAAAAACCCCCCCCCCCCCCCCCC
P3_COMMENT=Should fail
=
SEQUENCE_ID=template_mis_11
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo
=
SEQUENCE_ID=template_mis_12
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_INCLUDED_REGION=25,26
SEQUENCE_TEMPLATE=aTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGGc
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo plus former error in ignoring included_region offset
=
SEQUENCE_ID=template_mis_13
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_left_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=cTTGATGGAGAATTGCGCAGAGGGGcTTGATGGAGAATTGCGCAGAGGGc
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo
=
SEQUENCE_ID=template_mis_14
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=GCCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAT
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_11
=
SEQUENCE_ID=template_mis_15
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13
=
SEQUENCE_ID=template_mis_16
PRIMER_NUM_RETURN=1
PRIMER_EXPLAIN_FLAG=1
PRIMER_PICK_ANYWAY=1
PRIMER_MAX_POLY_X=40
PRIMER_MAX_SELF_END=100
PRIMER_WT_SIZE_GT=0
PRIMER_WT_SIZE_LT=0
PRIMER_WT_TM_GT=.05
PRIMER_WT_TM_LT=.05
PRIMER_MAX_GC=100
PRIMER_MAX_TM=100
PRIMER_TASK=pick_right_only
PRIMER_MAX_TEMPLATE_MISPRIMING=100
SEQUENCE_PRIMER_REVCOMP=cTTGATGGAGAATTGCGCAGAGGGGc
SEQUENCE_INCLUDED_REGION=24,26
SEQUENCE_TEMPLATE=GCCCTCTGCGCAATTCTCCATCAAGCCCCTCTGCGCAATTCTCCATCAAG
P3_COMMENT=Test off-by-one error in checking for mispriming on the same strand as the oligo; SEQUENCE is complements of template_mis_13
=
SEQUENCE_ID=short_align_target_1
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_SELF_ANY=0.0
PRIMER_WT_SELF_END=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_LIBRARY_MISPRIMING=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY=0.0
PRIMER_PAIR_WT_COMPL_END=0.0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_INTERNAL_MIN_SIZE=18
PRIMER_INTERNAL_OPT_SIZE=20
PRIMER_INTERNAL_MAX_SIZE=27
PRIMER_INTERNAL_MIN_TM=57.0
PRIMER_INTERNAL_OPT_TM=60.0
PRIMER_INTERNAL_MAX_TM=63.0
PRIMER_INTERNAL_MIN_GC=20.0
PRIMER_INTERNAL_MAX_GC=80.0
PRIMER_INTERNAL_MAX_SELF_ANY=12.00
PRIMER_INTERNAL_MAX_SELF_END=12.00
PRIMER_INTERNAL_MAX_NS_ACCEPTED=0
PRIMER_INTERNAL_MAX_POLY_X=5
PRIMER_INTERNAL_MISHYB_LIBRARY=
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00
PRIMER_INTERNAL_MIN_QUALITY=0
PRIMER_INTERNAL_SALT_MONOVALENT=50.0
PRIMER_INTERNAL_DNA_CONC=50.0
PRIMER_INTERNAL_SALT_DIVALENT=0.0
PRIMER_INTERNAL_DNTP_CONC=0.0
PRIMER_INTERNAL_WT_TM_LT=1.0
PRIMER_INTERNAL_WT_TM_GT=1.0
PRIMER_INTERNAL_WT_SIZE_LT=1.0
PRIMER_INTERNAL_WT_SIZE_GT=1.0
PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0
PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0
PRIMER_INTERNAL_WT_SELF_ANY=0.0
PRIMER_INTERNAL_WT_NUM_NS=0.0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0
PRIMER_INTERNAL_WT_SEQ_QUAL=0.0
PRIMER_TASK=pick_right_only
PRIMER_FIRST_BASE_INDEX=1
PRIMER_PICK_ANYWAY=1
P3_COMMENT=Test for very short target of alignment number 1
=
SEQUENCE_ID=short_align_target_2
PRIMER_EXPLAIN_FLAG=1
PRIMER_MISPRIMING_LIBRARY=
SEQUENCE_TEMPLATE=ACCAAGAAAGTAGTTGACAAAAAAATGAAAACCACTATTATTTAAATTACGGAAAAAAAAAAACCAATAAACAAAAAACATAATAAAAGAAATGAAAAAAAAGAATATTAGTAGAGCATACTGAATTGTTTTCAGAAAGAGAGTGAAAGGTAACAAAAATTTACCTACTTTTCCTTTTTATTTCTCTGGTTTCGTTTCTTTTTTTTCGGCCACCCTATTTATTTTTTCACTGCTTGTAAACAACGTCTCACTTCAATAGTGTCTCCTTCATGTACTGAGATACTGCTGCAAGCGAAATAT
PRIMER_PRODUCT_SIZE_RANGE=150-250 100-300 301-400 401-500 501-600 601-700 701-850 851-1000
PRIMER_NUM_RETURN=5
PRIMER_MAX_END_STABILITY=9.0
PRIMER_MAX_LIBRARY_MISPRIMING=12.00
PRIMER_PAIR_MAX_LIBRARY_MISPRIMING=24.00
PRIMER_MAX_TEMPLATE_MISPRIMING=12.00
PRIMER_PAIR_MAX_TEMPLATE_MISPRIMING=24.00
PRIMER_MIN_SIZE=18
PRIMER_OPT_SIZE=20
PRIMER_MAX_SIZE=27
PRIMER_MIN_TM=57.0
PRIMER_OPT_TM=60.0
PRIMER_MAX_TM=63.0
PRIMER_PAIR_MAX_DIFF_TM=100.0
PRIMER_TM_FORMULA=0
PRIMER_MIN_GC=20.0
PRIMER_MAX_GC=80.0
PRIMER_MAX_SELF_ANY=8.00
PRIMER_MAX_SELF_END=3.00
PRIMER_PAIR_MAX_COMPL_ANY=8.00
PRIMER_PAIR_MAX_COMPL_END=3.00
PRIMER_MAX_NS_ACCEPTED=0
PRIMER_MAX_POLY_X=5
PRIMER_OUTSIDE_PENALTY=0
PRIMER_GC_CLAMP=0
PRIMER_SALT_MONOVALENT=50.0
PRIMER_SALT_CORRECTIONS=0
PRIMER_SALT_DIVALENT=0.0
PRIMER_DNTP_CONC=0.0
PRIMER_DNA_CONC=50.0
PRIMER_LIBERAL_BASE=1
PRIMER_LIB_AMBIGUITY_CODES_CONSENSUS=0
PRIMER_MIN_QUALITY=0
PRIMER_MIN_END_QUALITY=0
PRIMER_QUALITY_RANGE_MIN=0
PRIMER_QUALITY_RANGE_MAX=100
PRIMER_WT_TM_LT=1.0
PRIMER_WT_TM_GT=1.0
PRIMER_WT_SIZE_LT=1.0
PRIMER_WT_SIZE_GT=1.0
PRIMER_WT_GC_PERCENT_LT=0.0
PRIMER_WT_GC_PERCENT_GT=0.0
PRIMER_WT_SELF_ANY=0.0
PRIMER_WT_SELF_END=0.0
PRIMER_WT_NUM_NS=0.0
PRIMER_WT_LIBRARY_MISPRIMING=0.0
PRIMER_WT_SEQ_QUAL=0.0
PRIMER_WT_END_QUAL=0.0
PRIMER_WT_POS_PENALTY=0.0
PRIMER_WT_END_STABILITY=0.0
PRIMER_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_LT=0.0
PRIMER_PAIR_WT_PRODUCT_SIZE_GT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_LT=0.0
PRIMER_PAIR_WT_PRODUCT_TM_GT=0.0
PRIMER_PAIR_WT_DIFF_TM=0.0
PRIMER_PAIR_WT_COMPL_ANY=0.0
PRIMER_PAIR_WT_COMPL_END=0.0
PRIMER_PAIR_WT_LIBRARY_MISPRIMING=0.0
PRIMER_PAIR_WT_PR_PENALTY=1.0
PRIMER_PAIR_WT_IO_PENALTY=0.0
PRIMER_PAIR_WT_TEMPLATE_MISPRIMING=0.0
PRIMER_INTERNAL_MIN_SIZE=18
PRIMER_INTERNAL_OPT_SIZE=20
PRIMER_INTERNAL_MAX_SIZE=27
PRIMER_INTERNAL_MIN_TM=57.0
PRIMER_INTERNAL_OPT_TM=60.0
PRIMER_INTERNAL_MAX_TM=63.0
PRIMER_INTERNAL_MIN_GC=20.0
PRIMER_INTERNAL_MAX_GC=80.0
PRIMER_INTERNAL_MAX_SELF_ANY=12.00
PRIMER_INTERNAL_MAX_SELF_END=12.00
PRIMER_INTERNAL_MAX_NS_ACCEPTED=0
PRIMER_INTERNAL_MAX_POLY_X=5
PRIMER_INTERNAL_MISHYB_LIBRARY=
PRIMER_INTERNAL_MAX_LIBRARY_MISHYB=12.00
PRIMER_INTERNAL_MIN_QUALITY=0
PRIMER_INTERNAL_SALT_MONOVALENT=50.0
PRIMER_INTERNAL_DNA_CONC=50.0
PRIMER_INTERNAL_SALT_DIVALENT=0.0
PRIMER_INTERNAL_DNTP_CONC=0.0
PRIMER_INTERNAL_WT_TM_LT=1.0
PRIMER_INTERNAL_WT_TM_GT=1.0
PRIMER_INTERNAL_WT_SIZE_LT=1.0
PRIMER_INTERNAL_WT_SIZE_GT=1.0
PRIMER_INTERNAL_WT_GC_PERCENT_LT=0.0
PRIMER_INTERNAL_WT_GC_PERCENT_GT=0.0
PRIMER_INTERNAL_WT_SELF_ANY=0.0
PRIMER_INTERNAL_WT_NUM_NS=0.0
PRIMER_INTERNAL_WT_LIBRARY_MISHYB=0.0
PRIMER_INTERNAL_WT_SEQ_QUAL=0.0
PRIMER_TASK=pick_left_only
PRIMER_FIRST_BASE_INDEX=1
PRIMER_PICK_ANYWAY=1
P3_COMMENT=Test for very short target of alignment number 1
=
